Mark Snyder

Bio-Rad

The first step in purification of an important class of therapeutic proteins, the polyclonal or monoclonal antibodies is their capture from plasma or tissue culture supernatants. Protein A-based media are by far the most common class of affinity products used for this purpose. In combination with ion exchange and ceramic hydroxyapatite chromatography, protein A-based media have been successfully used in the large-scale purification of numerous licensed mAb drugs.

Bio-Rad InPlace columns offer a unique solution for packing media because they are designed with low-shear slurry valves, which minimize damage to media such as ceramic hydroxyapatite. The InPlace column slurry valves are used for transferring media into and out of the column without removing the top flow adaptor. The valves are on the side of the column wall and do not interfere with flow dynamics.

Apatite-based media have been shown to be effective chromatographic supports for monoclonal antibody (mAb) purification. A two-step purification platform consisting of affinity medium capture followed by a CHT ceramic hydroxyapatite or CFT ceramic fluoroapatite media polishing step results in highly purified chimeric IgG by removing leached protein A, HCP, DNA and MAb aggregates

The first step in purification of an important class of therapeutic proteins, the polyclonal or monoclonal antibodies is their capture from plasma or tissue culture supernatants. Protein A-based media are by far the most common class of affinity products used for this purpose. In combination with ion exchange and ceramic hydroxyapatite chromatography, protein A-based media have been successfully used in the large-scale purification of numerous licensed MAb drugs.