Monoclonal Antibody Purification Using UNOsphere SUPrA Media
July 8, 2009
By: Xuemei He
,
Cheryl Aberin
,
Mark Snyder
The first step in purification of an important class of therapeutic proteins, the polyclonal or monoclonal antibodies is their capture from plasma or tissue culture supernatants. Protein A-based media are by far the most common class of affinity products used for this purpose. In combination with ion exchange and ceramic hydroxyapatite chromatography, protein A-based media have been successfully used in the large-scale purification of numerous licensed mAb drugs. |
Monoclonal Antibody Purification Using UNOsphere SUPrA Media-BioRad Whitepaper
June 15, 2009
By: Xuemei He
,
Cheryl Aberin
,
Jie He
,
Mark Snyder
The first step in purification of an important class of therapeutic proteins, the polyclonal or monoclonal antibodies is their capture from plasma or tissue culture supernatants. Protein A-based media are by far the most common class of affinity products used for this purpose. In combination with ion exchange and ceramic hydroxyapatite chromatography, protein A-based media have been successfully used in the large-scale purification of numerous licensed MAb drugs. |
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